GLB1 ELISA Kit

Catalog No. ABIN2043529

Human GLB1 ELISA Kit Colorimetric assay for quantification of Human GLB1.

Quick Overview for GLB1 ELISA Kit (ABIN2043529)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details GLB1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: Galactosidase Beta (GLb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Beta (GLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Beta (GLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Beta (GLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Galactosidase Beta (GLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Galactosidase beta (GLb)

Pathways: Glycosaminoglycan Metabolic Process