GDI1 ELISA Kit

Catalog No. ABIN2043539

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GDI1.

Quick Overview for GDI1 ELISA Kit (ABIN2043539)

Target: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GDI1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: GDP Dissociation Inhibitor 1 (GDI1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDP Dissociation Inhibitor 1 (GDI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of GDP Dissociation Inhibitor 1 (GDI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GDI1

Target: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))