GFRA1 ELISA Kit

Catalog No. ABIN2043543

This Human GFRA1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human GFRA1.

Quick Overview for GFRA1 ELISA Kit (ABIN2043543)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details GFRA1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.119 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: Glial Cell Line Derived Neurotrophic Factor Receptor alpha 1 (GFRa1)