GLP1R ELISA Kit

Catalog No. ABIN2043545

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GLP1R.

Quick Overview for GLP1R ELISA Kit (ABIN2043545)

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details GLP1R ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.118 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glucagon Like Peptide 1 Receptor (GLP1R) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 1 Receptor (GLP1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucagon Like Peptide 1 Receptor (GLP1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GLP1R

Target: GLP1R (Glucagon-Like Peptide 1 Receptor (GLP1R))

Alternative Name: Glucagon Like Peptide 1 Receptor (GLP1R)

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, cAMP Metabolic Process, Feeding Behaviour