Glucose-6-Phosphate Dehydrogenase ELISA Kit

Catalog No. ABIN2043549

This Mouse Glucose-6-Phosphate Dehydrogenase ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Glucose-6-Phosphate Dehydrogenase.

Quick Overview for Glucose-6-Phosphate Dehydrogenase ELISA Kit (ABIN2043549)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Glucose-6-Phosphate Dehydrogenase ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.137 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glucose 6 Phosphate Dehydrogenase (G6PD) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucose 6 Phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose 6 Phosphate Dehydrogenase (G6PD)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones