GAA ELISA Kit

Catalog No. ABIN2043555

This Mouse GAA ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse GAA.

Quick Overview for GAA ELISA Kit (ABIN2043555)

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details GAA ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 14.3 pg/mL

Application Details

Plate: Pre-coated

Protocol: Glucosidase Alpha, Acid (GaA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GAA

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Alternative Name: Glucosidase Alpha, Acid (GaA)

Pathways: Cellular Glucan Metabolic Process