GLCE ELISA Kit

Catalog No. ABIN2043557

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat GLCE.

Quick Overview for GLCE ELISA Kit (ABIN2043557)

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details GLCE ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.26 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glucuronic Acid Epimerase (GLCE) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucuronic Acid Epimerase (GLCE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucuronic Acid Epimerase (GLCE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucuronic Acid Epimerase (GLCE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucuronic Acid Epimerase (GLCE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GLCE

Target: GLCE (Glucuronic Acid Epimerase (GLCE))

Pathways: Glycosaminoglycan Metabolic Process