Glutathione Peroxidase 2 ELISA Kit

Catalog No. ABIN2043569

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Glutathione Peroxidase 2.

Quick Overview for Glutathione Peroxidase 2 ELISA Kit (ABIN2043569)

Target: Glutathione Peroxidase 2 (GPX2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Glutathione Peroxidase 2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.118 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glutathione Peroxidase 2, Gastrointestinal (GPX2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 2, Gastrointestinal (GPX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 2, Gastrointestinal (GPX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutathione Peroxidase 2, Gastrointestinal (GPX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Glutathione Peroxidase 2

Target: Glutathione Peroxidase 2 (GPX2)

Alternative Name: Glutathione Peroxidase 2, Gastrointestinal (GPX2)

Pathways: Thyroid Hormone Synthesis