GSTA4 ELISA Kit

Catalog No. ABIN2043573

This Mouse GSTA4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse GSTA4.

Quick Overview for GSTA4 ELISA Kit (ABIN2043573)

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GSTA4 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Glutathione S Transferase Alpha 4 (GSTa4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 4 (GSTa4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 4 (GSTa4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 4 (GSTa4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutathione S Transferase Alpha 4 (GSTa4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GSTA4

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))