GLO1 ELISA Kit
Quick Overview for GLO1 ELISA Kit (ABIN2043587)
Target
See all GLO1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 1.56 ng/mL
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Brand
- BioAssay™
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Sensitivity
- < 0.63 ng/mL
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Glyoxalase I (GLO1) ELISA Kit
GLO1 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Glyoxalase I (GLO1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyoxalase I (GLO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyoxalase I (GLO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyoxalase I (GLO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glyoxalase I (GLO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- GLO1 (Glyoxalase I (GLO1))
Target See all GLO1 ELISA Kits
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