GZMH ELISA Kit

Catalog No. ABIN2043595

Human GZMH ELISA Kit Colorimetric assay for quantification of Human GZMH.

Quick Overview for GZMH ELISA Kit (ABIN2043595)

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 pg/mL

Application: ELISA

Product Details GZMH ELISA Kit

Minimum Detection Limit: 1.56 pg/mL

Sensitivity: < 0.57 pg/mL

Application Details

Plate: Pre-coated

Protocol: Granzyme H (GZMH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme H (GZMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme H (GZMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme H (GZMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Granzyme H (GZMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GZMH

Target: GZMH (Granzyme H (Cathepsin G-Like 2, Protein H-CCPX) (GZMH))

Alternative Name: Granzyme H (GZMH)