GUCY1B3 ELISA Kit

Catalog No. ABIN2043607

Human GUCY1B3 ELISA Kit Colorimetric assay for quantification of Human GUCY1B3.

Quick Overview for GUCY1B3 ELISA Kit (ABIN2043607)

Target: GUCY1B3 (Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GUCY1B3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: Guanylate Cyclase 1 Beta 3 (GUCY1b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase 1 Beta 3 (GUCY1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Guanylate Cyclase 1 Beta 3 (GUCY1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GUCY1B3

Target: GUCY1B3 (Guanylate Cyclase 1, Soluble, beta 3 (GUCY1B3))

Alternative Name: Guanylate Cyclase 1 beta 3 (GUCY1b3)

Pathways: Transition Metal Ion Homeostasis