HSPA1B ELISA Kit

Catalog No. ABIN2043613

Cow HSPA1B ELISA Kit Colorimetric assay for quantification of Cow HSPA1B.

Quick Overview for HSPA1B ELISA Kit (ABIN2043613)

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details HSPA1B ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.53 ng/mL

Application Details

Plate: Pre-coated

Protocol: Heat Shock 70kDa Protein 1B (HSPA1B) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HSPA1B

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))