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HSP70 ELISA Kit

This Chicken HSP70 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chicken HSP70.
Catalog No. ABIN2043615

Quick Overview for HSP70 ELISA Kit (ABIN2043615)

Target

See all HSP70 ELISA Kits
HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity

  • 7
  • 6
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Chicken

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

1.56-100 ng/mL

Application

ELISA
  • Minimum Detection Limit

    1.56 ng/mL

    Sensitivity

    < 0.58 ng/mL
  • Plate

    Pre-coated

    Protocol

    Heat Shock Protein 70 (HSP70) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all HSP70 ELISA Kits

    HSP70 (Heat Shock Protein 70 (HSP70))
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