HSP70 ELISA Kit
Quick Overview for HSP70 ELISA Kit (ABIN2043615)
Target
See all HSP70 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
-
-
Minimum Detection Limit
- 1.56 ng/mL
-
Sensitivity
- < 0.58 ng/mL
-
-
-
-
Plate
- Pre-coated
-
Protocol
- Heat Shock Protein 70 (HSP70) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C/-20 °C
-
-
- HSP70 (Heat Shock Protein 70 (HSP70))
Target See all HSP70 ELISA Kits
-