HDGF ELISA Kit

Catalog No. ABIN2043637

Mouse HDGF ELISA Kit Colorimetric assay for quantification of Mouse HDGF.

Quick Overview for HDGF ELISA Kit (ABIN2043637)

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details HDGF ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 13.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: Hepatoma Derived Growth Factor (HDGF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatoma Derived Growth Factor (HDGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatoma Derived Growth Factor (HDGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatoma Derived Growth Factor (HDGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hepatoma Derived Growth Factor (HDGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HDGF

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Alternative Name: Hepatoma Derived Growth Factor (HDGF)

Pathways: ER-Nucleus Signaling