ChT ELISA Kit

Catalog No. ABIN2043643

Rat ChT ELISA Kit Colorimetric assay for quantification of Rat ChT.

Quick Overview for ChT ELISA Kit (ABIN2043643)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details ChT ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 28 pg/mL

Application Details

Plate: Pre-coated

Protocol: High Affinity Choline Transporter (ChT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter (ChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Affinity Choline Transporter (ChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Affinity Choline Transporter (ChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Affinity Choline Transporter (ChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))