IgG ELISA Kit

Catalog No. ABIN2043661

Cow IgG ELISA Kit Colorimetric assay for quantification of Cow IgG.

Quick Overview for IgG ELISA Kit (ABIN2043661)

Target: IgG

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details IgG ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.29 ng/mL

Application Details

Plate: Pre-coated

Protocol: High Sensitivity Immunoglobulin G (IgG) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G (IgG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G (IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G (IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IgG

Target: IgG

Alternative Name: Immunoglobulin G (IgG)

Target Type: Antibody