IL1A ELISA Kit

Catalog No. ABIN2043671

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat IL1A.

Quick Overview for IL1A ELISA Kit (ABIN2043671)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 pg/mL

Application: ELISA

Product Details IL1A ELISA Kit

Minimum Detection Limit: 0.78 pg/mL

Sensitivity: < 0.21 pg/mL

Application Details

Plate: Pre-coated

Protocol: High Sensitivity Interleukin 1 Alpha (IL1a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints