Nerve Growth Factor ELISA Kit
Quick Overview for Nerve Growth Factor ELISA Kit (ABIN2043685)
Target
See all Nerve Growth Factor (NGF) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.12 pg/mL
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Brand
- BioAssay™
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Sensitivity
- < 0.95 pg/mL
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Components
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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Nerve Growth Factor (NGF) ELISA Kit
NGF Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitHigh Sensitivity NGF Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Human Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
Nerve Growth Factor (NGF) ELISA KitWide Range NGF Reactivity: Rat Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- The Human Nerve Growth Factor (NGF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NGF in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Nerve Growth Factor (NGF)
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Pathways
- Regulation of Cell Size
Target See all Nerve Growth Factor (NGF) ELISA Kits
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