HRH1 ELISA Kit

Catalog No. ABIN2043691

Human HRH1 ELISA Kit Colorimetric assay for quantification of Human HRH1.

Quick Overview for HRH1 ELISA Kit (ABIN2043691)

Target: HRH1 (Histamine Receptor H1 (HRH1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details HRH1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.128 ng/mL

Application Details

Plate: Pre-coated

Protocol: Histamine Receptor H1 (HRH1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H1 (HRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H1 (HRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H1 (HRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histamine Receptor H1 (HRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Pathways: Regulation of Carbohydrate Metabolic Process