HRH4 ELISA Kit

Catalog No. ABIN2043695

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat HRH4.

Quick Overview for HRH4 ELISA Kit (ABIN2043695)

Target: HRH4 (Histamine Receptor H4 (HRH4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details HRH4 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Histamine Receptor H4 (HRH4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H4 (HRH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H4 (HRH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H4 (HRH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Histamine Receptor H4 (HRH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HRH4

Target: HRH4 (Histamine Receptor H4 (HRH4))