HTRA1 ELISA Kit

Catalog No. ABIN2043717

This Human HTRA1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human HTRA1.

Quick Overview for HTRA1 ELISA Kit (ABIN2043717)

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details HTRA1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 14.3 pg/mL

Components: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Human HtrA Serine Peptidase (HTRA1) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HTRA1 in human serum, plasma and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HTRA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HTRA1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HTRA1

Target: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Pathways: Growth Factor Binding