ISLR ELISA Kit

Catalog No. ABIN2043735

This Human ISLR ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ISLR.

Quick Overview for ISLR ELISA Kit (ABIN2043735)

Target: ISLR (Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ISLR ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.065 ng/mL

Application Details

Plate: Pre-coated

Protocol: Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ISLR

Target: ISLR (Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR))