Integrin Alpha2b ELISA Kit

Catalog No. ABIN2043767

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Integrin Alpha2b.

Quick Overview for Integrin Alpha2b ELISA Kit (ABIN2043767)

Target: Integrin Alpha2b (CD41)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Integrin Alpha2b ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Integrin Alpha 2B (ITGa2B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2B (ITGa2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 2B (ITGa2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 2B (ITGa2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Alpha 2B (ITGa2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: Integrin alpha 2B (ITGa2B)

Pathways: Integrin Complex