Integrin beta 5 ELISA Kit

Catalog No. ABIN2043775

Human Integrin beta 5 ELISA Kit Colorimetric assay for quantification of Human Integrin beta 5.

Quick Overview for Integrin beta 5 ELISA Kit (ABIN2043775)

Target: Integrin beta 5 (ITGB5)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Integrin beta 5 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.065 ng/mL

Application Details

Plate: Pre-coated

Protocol: Integrin Beta 5 (ITGb5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 5 (ITGb5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 5 (ITGb5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 5 (ITGb5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Beta 5 (ITGb5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Pathways: Integrin Complex