ITIH4 ELISA Kit

Catalog No. ABIN2043777

This Mouse ITIH4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse ITIH4.

Quick Overview for ITIH4 ELISA Kit (ABIN2043777)

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 ng/mL

Application: ELISA

Product Details ITIH4 ELISA Kit

Minimum Detection Limit: 7.81 ng/mL

Sensitivity: < 3.3 ng/mL

Application Details

Plate: Pre-coated

Protocol: Inter Alpha-Globulin Inhibitor H4 (ITIH4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ITIH4

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Alternative Name: Inter alpha-Globulin Inhibitor H4 (ITIH4)

Pathways: Glycosaminoglycan Metabolic Process