IRF4 ELISA Kit

Catalog No. ABIN2043783

This Rat IRF4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat IRF4.

Quick Overview for IRF4 ELISA Kit (ABIN2043783)

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details IRF4 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.133 ng/mL

Application Details

Plate: Pre-coated

Protocol: Interferon Regulatory Factor 4 (IRF4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IRF4

Target: IRF4 (Interferon Regulatory Factor 4 (IRF4))

Pathways: Chromatin Binding