Amylin/DAP ELISA Kit

Catalog No. ABIN2043821

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Amylin/DAP.

Quick Overview for Amylin/DAP ELISA Kit (ABIN2043821)

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 6.17-500 pg/mL

Application: ELISA

Product Details Amylin/DAP ELISA Kit

Minimum Detection Limit: 6.17 pg/mL

Brand: BioAssay™

Sensitivity: < 2.75 pg/mL

Application Details

Plate: Pre-coated

Protocol: Islet Amyloid Polypeptide (IAPP) BioAssay-ELISA Kit (Human) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Islet Amyloid Polypeptide (IAPP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Islet Amyloid Polypeptide (IAPP) and unlabeled Islet Amyloid Polypeptide (IAPP) (Standards or samples) with the pre-coated antibody specific for Islet Amyloid Polypeptide (IAPP). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Islet Amyloid Polypeptide (IAPP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Islet Amyloid Polypeptide (IAPP) in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Amylin/DAP

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Pathways: Hormone Activity, Feeding Behaviour