IARS ELISA Kit

Catalog No. ABIN2043823

Human IARS ELISA Kit Colorimetric assay for quantification of Human IARS.

Quick Overview for IARS ELISA Kit (ABIN2043823)

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details IARS ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.108 ng/mL

Application Details

Plate: Pre-coated

Protocol: Isoleucyl tRNA Synthetase (IARS) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IARS

Target: IARS (Isoleucyl-tRNA Synthetase (IARS))

Alternative Name: Isoleucyl tRNA Synthetase (IARS)