JKAMP ELISA Kit

Catalog No. ABIN2043829

Rat JKAMP ELISA Kit Colorimetric assay for quantification of Rat JKAMP.

Quick Overview for JKAMP ELISA Kit (ABIN2043829)

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details JKAMP ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.61 ng/mL

Application Details

Plate: Pre-coated

Protocol: JNK1/MAPK8 Associated Membrane Protein (JKAMP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JNK1/MAPK8 Associated Membrane Protein (JKAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of JNK1/MAPK8 Associated Membrane Protein (JKAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for JKAMP

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Alternative Name: JNK1/MAPK8 Associated Membrane Protein (JKAMP)