JAM2 ELISA Kit

Catalog No. ABIN2043831

This Human JAM2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human JAM2.

Quick Overview for JAM2 ELISA Kit (ABIN2043831)

Target: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Product Details JAM2 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Brand: BioAssay™

Analytical Method: Quantitative

Sensitivity: 13.3 pg/mL

Components: A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A (green), 1x120 µL
E: Detection Reagent B (red), 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Human Junctional Adhesion Molecule 2 (JAM2) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of JAM2 in human serum, plasma, tissue homogenates and other biological fluids.The Microtiter Strip provided in this kit has been pre-coated with an antibody specific to JAM2. Standards or samples are then added to the appropriate Microtiter Strip wells with a biotin-conjugated antibody specific to JAM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JAM2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for JAM2

Target: JAM2 (Junctional Adhesion Molecule 2 (JAM2))