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JAM2 ELISA Kit (Junctional Adhesion Molecule 2) ELISA Kit

JAM2 Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6730316
Plus shipping costs $45.00
96 tests
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Delivery in 21 to 31 Business Days
  • Target
    Detection Method
    Method Type
    Sandwich ELISA
    Mouse JAM-B/VE-JAM ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    This ELISA antibody pair recognizes Mouse JAM-B.
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target
    Alternative Name
    JAM-B (VE-JAM (JAM2 ELISA Kit Abstract)
    JAM2, cd322, jam-b, jamb, pro245, ve-jam, vejam, jam2, sb:cb1002, im:7155552, sc:d0813, si:ch211-160b11.5, LOC100227766, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM-2, JAM-B, Jcam2, VE-JAM, C21orf43, CD322, JAMB, PRO245, VEJAM, junctional adhesion molecule 2, junctional adhesion molecule 2a, junctional adhesion molecule 2b, junction adhesion molecule 2, JAM2, jam2, jam2a, jam2b, Jam2
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