LCT ELISA Kit

Catalog No. ABIN2043867

This Rat LCT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat LCT.

Quick Overview for LCT ELISA Kit (ABIN2043867)

Target: LCT (Lactase (LCT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details LCT ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: Lactase (LCT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactase (LCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactase (LCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactase (LCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactase (LCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for LCT

Target: LCT (Lactase (LCT))