LIFR ELISA Kit

Catalog No. ABIN2043887

This Colorimetric ELISA kit is designed for the quantitative measurement of Human LIFR.

Quick Overview for LIFR ELISA Kit (ABIN2043887)

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details LIFR ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Leukemia Inhibitory Factor Receptor (LIFR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor Receptor (LIFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor Receptor (LIFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Leukemia Inhibitory Factor Receptor (LIFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for LIFR

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Alternative Name: Leukemia Inhibitory Factor Receptor (LIFR)

Pathways: JAK-STAT Signaling, Growth Factor Binding