LIN28A ELISA Kit

Catalog No. ABIN2043891

This Mouse LIN28A ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse LIN28A.

Quick Overview for LIN28A ELISA Kit (ABIN2043891)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details LIN28A ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.049 ng/mL

Application Details

Plate: Pre-coated

Protocol: Lin-28 Homolog A (LIN28A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lin-28 Homolog A (LIN28A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lin-28 Homolog A (LIN28A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lin-28 Homolog A (LIN28A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lin-28 Homolog A (LIN28A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: Lin-28 Homolog A (LIN28A)

Pathways: Stem Cell Maintenance