MBL2 ELISA Kit

Catalog No. ABIN2043927

This Rat MBL2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat MBL2.

Quick Overview for MBL2 ELISA Kit (ABIN2043927)

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details MBL2 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.57 ng/mL

Application Details

Plate: Pre-coated

Protocol: Mannose Binding Lectin (MBL) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Binding Lectin (MBL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Binding Lectin (MBL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Binding Lectin (MBL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mannose Binding Lectin (MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MBL2

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Alternative Name: Mannose Binding Lectin (MBL)

Pathways: Complement System, Positive Regulation of Immune Effector Process