MAN2B1 ELISA Kit

Catalog No. ABIN2043931

Human MAN2B1 ELISA Kit Colorimetric assay for quantification of Human MAN2B1.

Quick Overview for MAN2B1 ELISA Kit (ABIN2043931)

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details MAN2B1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 14.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Mannosidase Alpha Class 2B Member 1 (MAN2B1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MAN2B1

Target: MAN2B1 (Mannosidase, Alpha, Class 2B, Member 1 (MAN2B1))

Alternative Name: Mannosidase alpha Class 2B Member 1 (MAN2B1)