MACC1 ELISA Kit

Catalog No. ABIN2043961

Human MACC1 ELISA Kit Colorimetric assay for quantification of Human MACC1.

Quick Overview for MACC1 ELISA Kit (ABIN2043961)

Target: MACC1 (Metastasis Associated in Colon Cancer 1 (MACC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details MACC1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.127 ng/mL

Application Details

Plate: Pre-coated

Protocol: Metastasis Associated In Colon Cancer 1 (MACC1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metastasis Associated In Colon Cancer 1 (MACC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metastasis Associated In Colon Cancer 1 (MACC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metastasis Associated In Colon Cancer 1 (MACC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Metastasis Associated In Colon Cancer 1 (MACC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MACC1

Target: MACC1 (Metastasis Associated in Colon Cancer 1 (MACC1))