MTAP ELISA Kit

Catalog No. ABIN2043971

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse MTAP.

Quick Overview for MTAP ELISA Kit (ABIN2043971)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MTAP ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Methylthioadenosine Phosphorylase (MTAP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process