Monoamine Oxidase A ELISA Kit

Catalog No. ABIN2043987

This Rat Monoamine Oxidase A ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat Monoamine Oxidase A.

Quick Overview for Monoamine Oxidase A ELISA Kit (ABIN2043987)

Target: Monoamine Oxidase A (MAOA)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125-8000 pg/mL

Application: ELISA

Product Details Monoamine Oxidase A ELISA Kit

Minimum Detection Limit: 125 pg/mL

Sensitivity: < 55 pg/mL

Application Details

Plate: Pre-coated

Protocol: Monoamine Oxidase A (MAOA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoamine Oxidase A (MAOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoamine Oxidase A (MAOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoamine Oxidase A (MAOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Monoamine Oxidase A (MAOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Monoamine Oxidase A

Target: Monoamine Oxidase A (MAOA)