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MBP ELISA Kit

This Rabbit MBP ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rabbit MBP.
Catalog No. ABIN2043999

Quick Overview for MBP ELISA Kit (ABIN2043999)

Target

See all MBP ELISA Kits
MBP (Myelin Basic Protein (MBP))

Reactivity

  • 6
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Rabbit

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.15-10 ng/mL

Application

ELISA
  • Minimum Detection Limit

    0.15 ng/mL

    Sensitivity

    < 0.055 ng/mL
  • Plate

    Pre-coated

    Protocol

    Myelin Basic Protein (MBP) BioAssay-ELISA Kit (Rabbit) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myelin Basic Protein (MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all MBP ELISA Kits

    MBP (Myelin Basic Protein (MBP))
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