CCDC177 ELISA Kit

Catalog No. ABIN2044003

Human CCDC177 ELISA Kit Colorimetric assay for quantification of Human CCDC177.

Quick Overview for CCDC177 ELISA Kit (ABIN2044003)

Target: CCDC177 (Coiled-Coil Domain Containing 177 (CCDC177))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CCDC177 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Myelin Proteolipid Protein Like Protein (PLPL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Proteolipid Protein Like Protein (PLPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Proteolipid Protein Like Protein (PLPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Proteolipid Protein Like Protein (PLPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myelin Proteolipid Protein Like Protein (PLPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCDC177

Target: CCDC177 (Coiled-Coil Domain Containing 177 (CCDC177))

Alternative Name: Myelin Proteolipid Protein Like Protein (PLPL)