MYL3/CMLC1 ELISA Kit

Catalog No. ABIN2044013

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MYL3/CMLC1.

Quick Overview for MYL3/CMLC1 ELISA Kit (ABIN2044013)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78-5000 pg/mL

Application: ELISA

Product Details MYL3/CMLC1 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Analytical Method: Quantitative

Sensitivity: < 28 pg/mL

Components: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Human Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYL3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYL3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Alternative Name: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3)