MYLK ELISA Kit

Catalog No. ABIN2044015

Rat MYLK ELISA Kit Colorimetric assay for quantification of Rat MYLK.

Quick Overview for MYLK ELISA Kit (ABIN2044015)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MYLK ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Components: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use.
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Rat Myosin Light Chain Kinase (MYLK) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYLK in rat tissue homogenates and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYLK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYLK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYLK in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))