NAPSA ELISA Kit

Catalog No. ABIN2044049

This Colorimetric ELISA kit is designed for the quantitative measurement of Human NAPSA.

Quick Overview for NAPSA ELISA Kit (ABIN2044049)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.87-3000 pg/mL

Application: ELISA

Product Details NAPSA ELISA Kit

Minimum Detection Limit: 46.87 pg/mL

Sensitivity: < 18.22 pg/mL

Application Details

Plate: Pre-coated

Protocol: Napsin A Aspartic Peptidase (NAPSA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Napsin A Aspartic Peptidase (NAPSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Napsin A Aspartic Peptidase (NAPSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Napsin A Aspartic Peptidase (NAPSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Napsin A Aspartic Peptidase (NAPSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis