NEFH ELISA Kit

Catalog No. ABIN2044075

Human NEFH ELISA Kit Colorimetric assay for quantification of Human NEFH.

Quick Overview for NEFH ELISA Kit (ABIN2044075)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details NEFH ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.2 pg/mL

Application Details

Plate: Pre-coated

Protocol: Neurofilament, Heavy Polypeptide (NEFH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Heavy Polypeptide (NEFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurofilament, Heavy Polypeptide (NEFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))