Neutrophil Alkaline Phosphatase ELISA Kit

Catalog No. ABIN2044089

This Human Neutrophil Alkaline Phosphatase ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Neutrophil Alkaline Phosphatase.

Quick Overview for Neutrophil Alkaline Phosphatase ELISA Kit (ABIN2044089)

Target: Neutrophil Alkaline Phosphatase (NAP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details Neutrophil Alkaline Phosphatase ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.57 ng/mL

Application Details

Plate: Pre-coated

Protocol: Neutrophil Alkaline Phosphatase (NAP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Alkaline Phosphatase (NAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Alkaline Phosphatase (NAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Alkaline Phosphatase (NAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neutrophil Alkaline Phosphatase (NAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Neutrophil Alkaline Phosphatase

Target: Neutrophil Alkaline Phosphatase (NAP)