NKAP ELISA Kit

Catalog No. ABIN2044093

Rat NKAP ELISA Kit Colorimetric assay for quantification of Rat NKAP.

Quick Overview for NKAP ELISA Kit (ABIN2044093)

Target: NKAP (NFKB Activating Protein (NKAP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details NKAP ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: NFKB Activating Protein (NKAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Activating Protein (NKAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Activating Protein (NKAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Activating Protein (NKAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NFKB Activating Protein (NKAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NKAP

Target: NKAP (NFKB Activating Protein (NKAP))

Pathways: Stem Cell Maintenance, The Global Phosphorylation Landscape of SARS-CoV-2 Infection