NFIB ELISA Kit

Catalog No. ABIN2044115

This Colorimetric ELISA kit is designed for the quantitative measurement of Human NFIB.

Quick Overview for NFIB ELISA Kit (ABIN2044115)

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details NFIB ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Nuclear Factor I/B (NFIB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B (NFIB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor I/B (NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B (NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Factor I/B (NFIB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))