NRF2 ELISA Kit

Catalog No. ABIN2044123

Rat NRF2 ELISA Kit, Colorimetric assay for quantification of Rat NRF2.

Quick Overview for NRF2 ELISA Kit (ABIN2044123)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details NRF2 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.5 pg/mL

Application Details

Plate: Pre-coated

Protocol: Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling